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Fast Protein Liquid Chromatography (FPLC)

Laboratory

Laboratory of Professor Élodie Boisselier


Department

Department of Ophthalmology and Otolaryngology – Head and Neck Surgery, Faculty of Medicine


Funding agency(ies)

CRSNG


Manufacturer (model)

Cytiva/GE Healthcare Life Sciences (ÄKTA pure)


Commissioning

2019


Equipment Description

The FPLC (Fast Protein Liquid Chromatography) system is designed for the rapid and automated purification of biomolecules. The available equipment is dedicated to analytical and preparative purification, as well as method development. It features a detection system at 280 nm. A wide range of purification columns can be used with this system, including affinity, ion-exchange, and size-exclusion chromatography columns. The system is connected to an automatic fraction collector and is controlled using the UNICORN software on a computer.


Technical Specifications

  • Flow rate: 0.001–25 mL/min

  • Operating pH range: 2–12

  • Detection: 280 nm

  • Fraction collectors


Areas of expertise

  • Column chromatography/protein, peptide, and nucleic acid purification


Conditions of Use

This service is available to academic and industrial collaborators. This technique requires the assistance of a research professional.


Rental Fees (CAD)


On-Campus

To be determined.


Off-Campus (Academic)

To be determined.


Industry

To be determined.


Reservations

Line Cantin

line.cantin@fmed.ulaval.ca

418 208-9712



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