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Gel Permeation Chromatography (GPC)

Laboratory

Nanopharmacology and Pharmaceutical Nanoscience


Department

Research Centre of the Centre hospitalier universitaire de Québec – Université Laval (CHUL)


Funding agency(ies)

Fondation canadienne pour l’innovation (FCI – Fonds des leaders John-R.-Evans (FLJR)


Manufacturer (model)

Agilent


Commissioning

2017


Equipment Description

Size Exclusion Chromatography (SEC) is an analytical technique used to characterize polymers in solution. This technique allows the determination of the number-average molar mass (Mn), weight-average molar mass (Mw), and polydispersity index (PDI) of materials. In an SEC column, polymer chains are separated according to their hydrodynamic diameter.

The mobile phase is THF. Measurements are relative to polystyrene standards (hydrophobic) only.


Technical Specifications

  • 2 Resipore Mixed columns, 4.6 × 300 mm

  • Mobile phase: THF

  • Detectors: UV–Vis, fluorescence, and refractive index

  • Polystyrene calibration: 162–364,000 g/mol

  • Analysis time: 25–45 minutes per measurement


Areas of expertise

  • Materials Science

  • Nanotechnology

  • Drug Delivery


Conditions of Use

Training on the instrument is required, or the instrument must be operated by a research professional. Available to academic and/or industrial collaborators.


Rental Fees (CAD)


On-Campus

$75/hour with assistance from a research professional


Off-Campus (Academic)

$100/hour with assistance from a research professional


Industry

$150/hour with assistance from a research professional


Reservations

Nicolas Bertrand

nicolas.bertrand@pha.ulaval.ca

418 656-2131 # 407042



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