Laboratory

Surface Engineering Laboratory (LIS)

Department

Department of Mining, Metallurgical and Materials Engineering

Funding agency(ies)

CRSNG

Manufacturer (model)

Carl Zeiss (LSM800 Axio Observer 7)

Commissioning

2018

Equipment Description

This Zeiss inverted microscope enables fluorescence imaging of a wide range of specimens, including opaque samples, gels, hydrogels, and tissues. The system can automatically acquire images at different time points, across different regions of the sample (tile), and at different depths (Z-stack).

The ZEISS LSM 800 is a high-end laser scanning confocal microscopy system. It is compact and versatile, and its configuration with three highly sensitive GaAsP detectors allows users to optimize the system for three key requirements in confocal microscopy: resolution, signal-to-noise ratio, and imaging speed.

The LSM 800’s linear scanning mode increases scanning speed while improving the signal-to-noise ratio by approximately 29%. Unlike other super-resolution techniques, it retains the imaging and optical sectioning capabilities of confocal systems. The LSM 800 can be configured according to specific research needs and adapted to future requirements through its various available options.

Technical Specifications

Objectives: 5X, 10X, 20X, 20X LD, and 40X (oil immersion)

• Laser: Diode lasers

• Detectors: Highly sensitive GaAsP detectors

• Automated stage

• Resolution: 4 × 1 to 6.1 × 6.1 pixels

• Speed: Up to 8 frames/s at 512 × 512 pixels

  
  

Areas of expertise

• High-resolution confocal microscopy

• 3D fluorescence imaging

• Imaging of cultured cells

• Tissue imaging

  
  

Other Related Equipment

• Bright-field and fluorescence microscopes

• Scanning microscope

  
  

Conditions of Use

This service is available to academic and industrial collaborators. This technique requires the presence of a research professional to perform the analysis.

Rental Fees (CAD)

On-Campus

To be determined

Off-Campus (Academic)

To be determined

Industry

To be determined

Reservations

Andrée-Anne Guay Bégin, M.Sc.

andree-anne.guay-begin@crchudequebec.ulaval.ca

418-525-4444 poste 52233